up-regulation of integrinsn α2β1 and α3β1 expression in human foreskin fibroblast cells after in-vitro infection with herpes simplex virus type 1
نویسندگان
چکیده
the interaction of herpes simplex virus type 1 (hsv-1) with human fetal foreskin fibroblast (hfff) cell was studied using a recent isolate of hsv-1 which was propagated in hep-2 cells. hfff cells were challenged with hsv-1 with a multiplicity of infection (moi) of 1 virus/cell for 24 hours. flow cytometric analysis demonstrated that hsv-1 challenged hfff cells expressed increased levels of α2β1, α3β1 (a collagen and laminin receptor) and lfa-1 (a β2 integrin, ligand for intercellular adhesion molecule-1, icam-1) integrins without a significant change in the expression of α5b1 (a fibronectin receptor) as compared to the mock-challenged cells. in addition, virus challenged hfff cells showed increased expression of icam-1 and hla class i antigen when compared to mock-infected cells. these results suggest that hsv-1 is capable of inducing functional changes in human foreskin fibroblast cells. the altered expression of cell-matrix and cell-cell adhesion molecules resulting from hsv-1 infection could contribute to the immunopathological diseases associated with hsv-1 infection in vivo.
منابع مشابه
Up-Regulation of Integrinsn α2β1 and α3β1 Expression in Human Foreskin Fibroblast Cells after In-Vitro Infection with Herpes Simplex Virus Type 1
The interaction of Herpes Simplex Virus type 1 (HSV-1) with human fetal foreskin fibroblast (HFFF) cell was studied using a recent isolate of HSV-1 which was propagated in Hep-2 cells. HFFF cells were challenged with HSV-1 with a multiplicity of infection (MOI) of 1 virus/cell for 24 hours. Flow cytometric analysis demonstrated that HSV-1 challenged HFFF cells expressed increased levels of α2β1...
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عنوان ژورنال:
iranian biomedical journalجلد ۵، شماره ۲، صفحات ۵۵-۵۹
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